Msh2 Knockout cell line (B16-F10)

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Msh2 Knockout cell line (B16-F10) Order

Msh2 Knockout cell line (B16-F10)
Catalog#
YKO-M208
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Msh2 Knockout cell line (B16-F10)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Msh2 Knockout cell line (B16-F10)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Gene Name
Msh2
Gene ID
17685
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 1 (based on transcript Msh2-201)
Validation: PCR+Sanger Sequencing
Msh2 Knockout cell line (B16-F10) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:B16-F10
STR Info (Cell bank)
Cell Line:B16-F10
Allele1Allele2Allele3Allele1Allele2Allele3
4-2 237.03
【20.3】
241.13
【21.3】
20.321.3
5-5 343.51
【16】
359.57
【20】
16.020.0
6-4 299.35
【18】
303.47
【19】
18.019.0
6-7 345.99
【15】
15.0
9-2 232.84
【18】
236.94
【19】
18.019.0
12-1 229.14
【17】
233.26
【18】
17.018.0
15-3 200.43
【22.3】
204.54
【23.3】
22.323.3
18-3 147.05
【15】
151.1
【16】
15.016.0
X-1 411.87
【28】
28.0
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Msh2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the B16-F10 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Msh2 gene knockout Msh2 Knockout cell line (B16-F10) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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